polyclonal rabbit anti rpl7a Search Results


rrna antibody (y10b) - bsa free  (Bio-Techne corporation)
93
Bio-Techne corporation rrna antibody (y10b) - bsa free
Rrna Antibody (Y10b) Bsa Free, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rrna antibody (y10b) - bsa free/product/Bio-Techne corporation
Average 93 stars, based on 1 article reviews
rrna antibody (y10b) - bsa free - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
rabbit anti rpl7a e109  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc rabbit anti rpl7a e109
Fig. 2. TAOK2β associates with the polyribosome complex, and its deficiency enhances general translation and protein synthesis. (A) Immunoblots of polysome fractions showing the presence or absence of TAOK2β across all polysome fractions isolated from Taok2+/+ (top) or Taok2−/− (bottom) mouse cortex. PABP1, <t>RPL7a,</t> and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were used as positive and negative controls, respectively, to prove polysome preparation efficiency. (B) Polysome profiles from Taok2+/+ and Taok2−/− mouse cortices. (C) Quantification of polysome profiles in Taok2−/−, Taok2+/−, and Taok2+/+ mice cortices. Taok2+/+, n = 13; Taok2+/−, n = 8; Taok2−/−, n = 12; scanning electron microscopy (SEM) error bars, ordinary one-way analysis of variance (ANOVA) (P = 0.0251) followed by Dunnett’s multiple com- parisons test (*P < 0.05). (D) Immunostainings of acute cortical slices from the prefrontal cortex of Taok2+/+ and Taok2−/− mice analyzed by SUnSET assay. WT slices pre- treated with the translation inhibitor cycloheximide (WT + CHX) verifies the specificity of the puromycin antibody signal. (E) Immunoblot from acute cortical slices analyzed by SUnSET assay. (F) Quantification from (E). Taok2+/+, n = 5; Taok2+/−, n = 6; Taok2−/−, n = 5; SEM error bars, ordinary one-way ANOVA (P = 0.0167) followed by Dunnett’s multiple comparisons test (*P < 0.05). (G) Immunostainings of primary neurons (7DIV) analyzed by SUnSET assay. Cultured WT neurons were pretreated with CHX (WT + CHX). (H and I) Quantifications of immunofluorescent SUnSET assay of 7DIV neurons or 21DIV. 7DIV cells: Taok2+/+, n = 236 (five embryos); Taok2+/−, n = 113 (four embryos); Taok2−/−, n = 166 (four embryos). 21DIV cells: Taok2+/+ (three embryos), n = 81; Taok2+/−, n = 73 (two embryos); Taok2−/−, n = 79 (three embryos). SEM error bars, ordinary one-way ANOVA (P < 0.0001) followed by Dunnett’s multiple comparisons test (****P < 0.0001). Scale bars, 10 μm [(D) and (G)].
Rabbit Anti Rpl7a E109, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti rpl7a e109/product/Cell Signaling Technology Inc
Average 94 stars, based on 1 article reviews
rabbit anti rpl7a e109 - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
polyclonal rabbit anti-rpl7a antibodies  (Bethyl)
90
Bethyl polyclonal rabbit anti-rpl7a antibodies
Fig. 2. TAOK2β associates with the polyribosome complex, and its deficiency enhances general translation and protein synthesis. (A) Immunoblots of polysome fractions showing the presence or absence of TAOK2β across all polysome fractions isolated from Taok2+/+ (top) or Taok2−/− (bottom) mouse cortex. PABP1, <t>RPL7a,</t> and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were used as positive and negative controls, respectively, to prove polysome preparation efficiency. (B) Polysome profiles from Taok2+/+ and Taok2−/− mouse cortices. (C) Quantification of polysome profiles in Taok2−/−, Taok2+/−, and Taok2+/+ mice cortices. Taok2+/+, n = 13; Taok2+/−, n = 8; Taok2−/−, n = 12; scanning electron microscopy (SEM) error bars, ordinary one-way analysis of variance (ANOVA) (P = 0.0251) followed by Dunnett’s multiple com- parisons test (*P < 0.05). (D) Immunostainings of acute cortical slices from the prefrontal cortex of Taok2+/+ and Taok2−/− mice analyzed by SUnSET assay. WT slices pre- treated with the translation inhibitor cycloheximide (WT + CHX) verifies the specificity of the puromycin antibody signal. (E) Immunoblot from acute cortical slices analyzed by SUnSET assay. (F) Quantification from (E). Taok2+/+, n = 5; Taok2+/−, n = 6; Taok2−/−, n = 5; SEM error bars, ordinary one-way ANOVA (P = 0.0167) followed by Dunnett’s multiple comparisons test (*P < 0.05). (G) Immunostainings of primary neurons (7DIV) analyzed by SUnSET assay. Cultured WT neurons were pretreated with CHX (WT + CHX). (H and I) Quantifications of immunofluorescent SUnSET assay of 7DIV neurons or 21DIV. 7DIV cells: Taok2+/+, n = 236 (five embryos); Taok2+/−, n = 113 (four embryos); Taok2−/−, n = 166 (four embryos). 21DIV cells: Taok2+/+ (three embryos), n = 81; Taok2+/−, n = 73 (two embryos); Taok2−/−, n = 79 (three embryos). SEM error bars, ordinary one-way ANOVA (P < 0.0001) followed by Dunnett’s multiple comparisons test (****P < 0.0001). Scale bars, 10 μm [(D) and (G)].
Polyclonal Rabbit Anti Rpl7a Antibodies, supplied by Bethyl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal rabbit anti-rpl7a antibodies/product/Bethyl
Average 90 stars, based on 1 article reviews
polyclonal rabbit anti-rpl7a antibodies - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
polyclonal rabbit anti rpl7a  (Danaher Inc)
86
Danaher Inc polyclonal rabbit anti rpl7a
Fig. 2. TAOK2β associates with the polyribosome complex, and its deficiency enhances general translation and protein synthesis. (A) Immunoblots of polysome fractions showing the presence or absence of TAOK2β across all polysome fractions isolated from Taok2+/+ (top) or Taok2−/− (bottom) mouse cortex. PABP1, <t>RPL7a,</t> and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were used as positive and negative controls, respectively, to prove polysome preparation efficiency. (B) Polysome profiles from Taok2+/+ and Taok2−/− mouse cortices. (C) Quantification of polysome profiles in Taok2−/−, Taok2+/−, and Taok2+/+ mice cortices. Taok2+/+, n = 13; Taok2+/−, n = 8; Taok2−/−, n = 12; scanning electron microscopy (SEM) error bars, ordinary one-way analysis of variance (ANOVA) (P = 0.0251) followed by Dunnett’s multiple com- parisons test (*P < 0.05). (D) Immunostainings of acute cortical slices from the prefrontal cortex of Taok2+/+ and Taok2−/− mice analyzed by SUnSET assay. WT slices pre- treated with the translation inhibitor cycloheximide (WT + CHX) verifies the specificity of the puromycin antibody signal. (E) Immunoblot from acute cortical slices analyzed by SUnSET assay. (F) Quantification from (E). Taok2+/+, n = 5; Taok2+/−, n = 6; Taok2−/−, n = 5; SEM error bars, ordinary one-way ANOVA (P = 0.0167) followed by Dunnett’s multiple comparisons test (*P < 0.05). (G) Immunostainings of primary neurons (7DIV) analyzed by SUnSET assay. Cultured WT neurons were pretreated with CHX (WT + CHX). (H and I) Quantifications of immunofluorescent SUnSET assay of 7DIV neurons or 21DIV. 7DIV cells: Taok2+/+, n = 236 (five embryos); Taok2+/−, n = 113 (four embryos); Taok2−/−, n = 166 (four embryos). 21DIV cells: Taok2+/+ (three embryos), n = 81; Taok2+/−, n = 73 (two embryos); Taok2−/−, n = 79 (three embryos). SEM error bars, ordinary one-way ANOVA (P < 0.0001) followed by Dunnett’s multiple comparisons test (****P < 0.0001). Scale bars, 10 μm [(D) and (G)].
Polyclonal Rabbit Anti Rpl7a, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal rabbit anti rpl7a/product/Danaher Inc
Average 86 stars, based on 1 article reviews
polyclonal rabbit anti rpl7a - by Bioz Stars, 2026-02
86/100 stars
  Buy from Supplier
anti rpl7a  (Proteintech)
93
Proteintech anti rpl7a
Fig. 2. TAOK2β associates with the polyribosome complex, and its deficiency enhances general translation and protein synthesis. (A) Immunoblots of polysome fractions showing the presence or absence of TAOK2β across all polysome fractions isolated from Taok2+/+ (top) or Taok2−/− (bottom) mouse cortex. PABP1, <t>RPL7a,</t> and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were used as positive and negative controls, respectively, to prove polysome preparation efficiency. (B) Polysome profiles from Taok2+/+ and Taok2−/− mouse cortices. (C) Quantification of polysome profiles in Taok2−/−, Taok2+/−, and Taok2+/+ mice cortices. Taok2+/+, n = 13; Taok2+/−, n = 8; Taok2−/−, n = 12; scanning electron microscopy (SEM) error bars, ordinary one-way analysis of variance (ANOVA) (P = 0.0251) followed by Dunnett’s multiple com- parisons test (*P < 0.05). (D) Immunostainings of acute cortical slices from the prefrontal cortex of Taok2+/+ and Taok2−/− mice analyzed by SUnSET assay. WT slices pre- treated with the translation inhibitor cycloheximide (WT + CHX) verifies the specificity of the puromycin antibody signal. (E) Immunoblot from acute cortical slices analyzed by SUnSET assay. (F) Quantification from (E). Taok2+/+, n = 5; Taok2+/−, n = 6; Taok2−/−, n = 5; SEM error bars, ordinary one-way ANOVA (P = 0.0167) followed by Dunnett’s multiple comparisons test (*P < 0.05). (G) Immunostainings of primary neurons (7DIV) analyzed by SUnSET assay. Cultured WT neurons were pretreated with CHX (WT + CHX). (H and I) Quantifications of immunofluorescent SUnSET assay of 7DIV neurons or 21DIV. 7DIV cells: Taok2+/+, n = 236 (five embryos); Taok2+/−, n = 113 (four embryos); Taok2−/−, n = 166 (four embryos). 21DIV cells: Taok2+/+ (three embryos), n = 81; Taok2+/−, n = 73 (two embryos); Taok2−/−, n = 79 (three embryos). SEM error bars, ordinary one-way ANOVA (P < 0.0001) followed by Dunnett’s multiple comparisons test (****P < 0.0001). Scale bars, 10 μm [(D) and (G)].
Anti Rpl7a, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti rpl7a/product/Proteintech
Average 93 stars, based on 1 article reviews
anti rpl7a - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
anti rpl7a  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc anti rpl7a
Fig. 2. TAOK2β associates with the polyribosome complex, and its deficiency enhances general translation and protein synthesis. (A) Immunoblots of polysome fractions showing the presence or absence of TAOK2β across all polysome fractions isolated from Taok2+/+ (top) or Taok2−/− (bottom) mouse cortex. PABP1, <t>RPL7a,</t> and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were used as positive and negative controls, respectively, to prove polysome preparation efficiency. (B) Polysome profiles from Taok2+/+ and Taok2−/− mouse cortices. (C) Quantification of polysome profiles in Taok2−/−, Taok2+/−, and Taok2+/+ mice cortices. Taok2+/+, n = 13; Taok2+/−, n = 8; Taok2−/−, n = 12; scanning electron microscopy (SEM) error bars, ordinary one-way analysis of variance (ANOVA) (P = 0.0251) followed by Dunnett’s multiple com- parisons test (*P < 0.05). (D) Immunostainings of acute cortical slices from the prefrontal cortex of Taok2+/+ and Taok2−/− mice analyzed by SUnSET assay. WT slices pre- treated with the translation inhibitor cycloheximide (WT + CHX) verifies the specificity of the puromycin antibody signal. (E) Immunoblot from acute cortical slices analyzed by SUnSET assay. (F) Quantification from (E). Taok2+/+, n = 5; Taok2+/−, n = 6; Taok2−/−, n = 5; SEM error bars, ordinary one-way ANOVA (P = 0.0167) followed by Dunnett’s multiple comparisons test (*P < 0.05). (G) Immunostainings of primary neurons (7DIV) analyzed by SUnSET assay. Cultured WT neurons were pretreated with CHX (WT + CHX). (H and I) Quantifications of immunofluorescent SUnSET assay of 7DIV neurons or 21DIV. 7DIV cells: Taok2+/+, n = 236 (five embryos); Taok2+/−, n = 113 (four embryos); Taok2−/−, n = 166 (four embryos). 21DIV cells: Taok2+/+ (three embryos), n = 81; Taok2+/−, n = 73 (two embryos); Taok2−/−, n = 79 (three embryos). SEM error bars, ordinary one-way ANOVA (P < 0.0001) followed by Dunnett’s multiple comparisons test (****P < 0.0001). Scale bars, 10 μm [(D) and (G)].
Anti Rpl7a, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti rpl7a/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
anti rpl7a - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
anti rpl7a antibodies  (Bethyl)
93
Bethyl anti rpl7a antibodies
Fig. 2. TAOK2β associates with the polyribosome complex, and its deficiency enhances general translation and protein synthesis. (A) Immunoblots of polysome fractions showing the presence or absence of TAOK2β across all polysome fractions isolated from Taok2+/+ (top) or Taok2−/− (bottom) mouse cortex. PABP1, <t>RPL7a,</t> and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were used as positive and negative controls, respectively, to prove polysome preparation efficiency. (B) Polysome profiles from Taok2+/+ and Taok2−/− mouse cortices. (C) Quantification of polysome profiles in Taok2−/−, Taok2+/−, and Taok2+/+ mice cortices. Taok2+/+, n = 13; Taok2+/−, n = 8; Taok2−/−, n = 12; scanning electron microscopy (SEM) error bars, ordinary one-way analysis of variance (ANOVA) (P = 0.0251) followed by Dunnett’s multiple com- parisons test (*P < 0.05). (D) Immunostainings of acute cortical slices from the prefrontal cortex of Taok2+/+ and Taok2−/− mice analyzed by SUnSET assay. WT slices pre- treated with the translation inhibitor cycloheximide (WT + CHX) verifies the specificity of the puromycin antibody signal. (E) Immunoblot from acute cortical slices analyzed by SUnSET assay. (F) Quantification from (E). Taok2+/+, n = 5; Taok2+/−, n = 6; Taok2−/−, n = 5; SEM error bars, ordinary one-way ANOVA (P = 0.0167) followed by Dunnett’s multiple comparisons test (*P < 0.05). (G) Immunostainings of primary neurons (7DIV) analyzed by SUnSET assay. Cultured WT neurons were pretreated with CHX (WT + CHX). (H and I) Quantifications of immunofluorescent SUnSET assay of 7DIV neurons or 21DIV. 7DIV cells: Taok2+/+, n = 236 (five embryos); Taok2+/−, n = 113 (four embryos); Taok2−/−, n = 166 (four embryos). 21DIV cells: Taok2+/+ (three embryos), n = 81; Taok2+/−, n = 73 (two embryos); Taok2−/−, n = 79 (three embryos). SEM error bars, ordinary one-way ANOVA (P < 0.0001) followed by Dunnett’s multiple comparisons test (****P < 0.0001). Scale bars, 10 μm [(D) and (G)].
Anti Rpl7a Antibodies, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti rpl7a antibodies/product/Bethyl
Average 93 stars, based on 1 article reviews
anti rpl7a antibodies - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
anti rpl7a  (ABclonal Biotechnology)
90
ABclonal Biotechnology anti rpl7a
Pre‐rRNPs colocalize with TopBP1 at DSBs. A) Pre‐rRNA colocalizes with TopBP1 at DSBs. Following 10 Gy of IR treatment on HCT116 cells, RNA FISH with pre‐rRNA probes and IF with anti‐TopBP1 antibody were performed. B–D) Pre‐rRNA‐associated proteins colocalize with TopBP1 at DSBs. Following 10 Gy of IR, IF was performed with anti‐TopBP1 and <t>anti‐RPL7A</t> (B), or anti‐RPS3 (C), or anti‐FBL antibodies (D). E) Pre‐rRNPs localize at I‐SceI‐induced DSB. I‐SceI‐mediated DSB was induced by Dox and TA treatment in the HCT116 cells harboring a solo I‐SceI site. Then, ChIP assay with the indicated antibodies was performed. Data are represented as mean ± SD as indicated from three independent experiments. F) TopBP1 localizes at the unsynapsed region of the XY body. Meiotic spread was examined with anti‐TopBP1 antibody. Sycp3 was a surrogate marker of the unsynapsed axis of the XY body in pachytene cells. G) Pre‐rRNA localizes at the unsynapsed region. Pre‐rRNA was examined by RNA FISH with pre‐rRNA probes. H–J) Pre‐rRNA‐associated proteins localize at the unsynapsed region. Meiotic spreads were stained with anti‐Sycp3 and anti‐RPL7A (H), anti‐RPS3 (I), or anti‐FBL antibodies (J). The relative intensity of fluorescence signals is analyzed. Two‐tailed Student's t ‐test is used to determine statistical significance. *** p < 0.001, versus control groups. Scale bars, 10 µm.
Anti Rpl7a, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti rpl7a/product/ABclonal Biotechnology
Average 90 stars, based on 1 article reviews
anti rpl7a - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
antibody anti-rps6  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc antibody anti-rps6
Pre‐rRNPs colocalize with TopBP1 at DSBs. A) Pre‐rRNA colocalizes with TopBP1 at DSBs. Following 10 Gy of IR treatment on HCT116 cells, RNA FISH with pre‐rRNA probes and IF with anti‐TopBP1 antibody were performed. B–D) Pre‐rRNA‐associated proteins colocalize with TopBP1 at DSBs. Following 10 Gy of IR, IF was performed with anti‐TopBP1 and <t>anti‐RPL7A</t> (B), or anti‐RPS3 (C), or anti‐FBL antibodies (D). E) Pre‐rRNPs localize at I‐SceI‐induced DSB. I‐SceI‐mediated DSB was induced by Dox and TA treatment in the HCT116 cells harboring a solo I‐SceI site. Then, ChIP assay with the indicated antibodies was performed. Data are represented as mean ± SD as indicated from three independent experiments. F) TopBP1 localizes at the unsynapsed region of the XY body. Meiotic spread was examined with anti‐TopBP1 antibody. Sycp3 was a surrogate marker of the unsynapsed axis of the XY body in pachytene cells. G) Pre‐rRNA localizes at the unsynapsed region. Pre‐rRNA was examined by RNA FISH with pre‐rRNA probes. H–J) Pre‐rRNA‐associated proteins localize at the unsynapsed region. Meiotic spreads were stained with anti‐Sycp3 and anti‐RPL7A (H), anti‐RPS3 (I), or anti‐FBL antibodies (J). The relative intensity of fluorescence signals is analyzed. Two‐tailed Student's t ‐test is used to determine statistical significance. *** p < 0.001, versus control groups. Scale bars, 10 µm.
Antibody Anti Rps6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody anti-rps6/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
antibody anti-rps6 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
rpl7a  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc rpl7a
Pre‐rRNPs colocalize with TopBP1 at DSBs. A) Pre‐rRNA colocalizes with TopBP1 at DSBs. Following 10 Gy of IR treatment on HCT116 cells, RNA FISH with pre‐rRNA probes and IF with anti‐TopBP1 antibody were performed. B–D) Pre‐rRNA‐associated proteins colocalize with TopBP1 at DSBs. Following 10 Gy of IR, IF was performed with anti‐TopBP1 and <t>anti‐RPL7A</t> (B), or anti‐RPS3 (C), or anti‐FBL antibodies (D). E) Pre‐rRNPs localize at I‐SceI‐induced DSB. I‐SceI‐mediated DSB was induced by Dox and TA treatment in the HCT116 cells harboring a solo I‐SceI site. Then, ChIP assay with the indicated antibodies was performed. Data are represented as mean ± SD as indicated from three independent experiments. F) TopBP1 localizes at the unsynapsed region of the XY body. Meiotic spread was examined with anti‐TopBP1 antibody. Sycp3 was a surrogate marker of the unsynapsed axis of the XY body in pachytene cells. G) Pre‐rRNA localizes at the unsynapsed region. Pre‐rRNA was examined by RNA FISH with pre‐rRNA probes. H–J) Pre‐rRNA‐associated proteins localize at the unsynapsed region. Meiotic spreads were stained with anti‐Sycp3 and anti‐RPL7A (H), anti‐RPS3 (I), or anti‐FBL antibodies (J). The relative intensity of fluorescence signals is analyzed. Two‐tailed Student's t ‐test is used to determine statistical significance. *** p < 0.001, versus control groups. Scale bars, 10 µm.
Rpl7a, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rpl7a/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
rpl7a - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
antibody anti-rps3  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc antibody anti-rps3
Pre‐rRNPs colocalize with TopBP1 at DSBs. A) Pre‐rRNA colocalizes with TopBP1 at DSBs. Following 10 Gy of IR treatment on HCT116 cells, RNA FISH with pre‐rRNA probes and IF with anti‐TopBP1 antibody were performed. B–D) Pre‐rRNA‐associated proteins colocalize with TopBP1 at DSBs. Following 10 Gy of IR, IF was performed with anti‐TopBP1 and <t>anti‐RPL7A</t> (B), or anti‐RPS3 (C), or anti‐FBL antibodies (D). E) Pre‐rRNPs localize at I‐SceI‐induced DSB. I‐SceI‐mediated DSB was induced by Dox and TA treatment in the HCT116 cells harboring a solo I‐SceI site. Then, ChIP assay with the indicated antibodies was performed. Data are represented as mean ± SD as indicated from three independent experiments. F) TopBP1 localizes at the unsynapsed region of the XY body. Meiotic spread was examined with anti‐TopBP1 antibody. Sycp3 was a surrogate marker of the unsynapsed axis of the XY body in pachytene cells. G) Pre‐rRNA localizes at the unsynapsed region. Pre‐rRNA was examined by RNA FISH with pre‐rRNA probes. H–J) Pre‐rRNA‐associated proteins localize at the unsynapsed region. Meiotic spreads were stained with anti‐Sycp3 and anti‐RPL7A (H), anti‐RPS3 (I), or anti‐FBL antibodies (J). The relative intensity of fluorescence signals is analyzed. Two‐tailed Student's t ‐test is used to determine statistical significance. *** p < 0.001, versus control groups. Scale bars, 10 µm.
Antibody Anti Rps3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody anti-rps3/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
antibody anti-rps3 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
scp3/sycp3 antibody - bsa free  (Bio-Techne corporation)
93
Bio-Techne corporation scp3/sycp3 antibody - bsa free
Pre‐rRNPs colocalize with TopBP1 at DSBs. A) Pre‐rRNA colocalizes with TopBP1 at DSBs. Following 10 Gy of IR treatment on HCT116 cells, RNA FISH with pre‐rRNA probes and IF with anti‐TopBP1 antibody were performed. B–D) Pre‐rRNA‐associated proteins colocalize with TopBP1 at DSBs. Following 10 Gy of IR, IF was performed with anti‐TopBP1 and <t>anti‐RPL7A</t> (B), or anti‐RPS3 (C), or anti‐FBL antibodies (D). E) Pre‐rRNPs localize at I‐SceI‐induced DSB. I‐SceI‐mediated DSB was induced by Dox and TA treatment in the HCT116 cells harboring a solo I‐SceI site. Then, ChIP assay with the indicated antibodies was performed. Data are represented as mean ± SD as indicated from three independent experiments. F) TopBP1 localizes at the unsynapsed region of the XY body. Meiotic spread was examined with anti‐TopBP1 antibody. Sycp3 was a surrogate marker of the unsynapsed axis of the XY body in pachytene cells. G) Pre‐rRNA localizes at the unsynapsed region. Pre‐rRNA was examined by RNA FISH with pre‐rRNA probes. H–J) Pre‐rRNA‐associated proteins localize at the unsynapsed region. Meiotic spreads were stained with anti‐Sycp3 and anti‐RPL7A (H), anti‐RPS3 (I), or anti‐FBL antibodies (J). The relative intensity of fluorescence signals is analyzed. Two‐tailed Student's t ‐test is used to determine statistical significance. *** p < 0.001, versus control groups. Scale bars, 10 µm.
Scp3/Sycp3 Antibody Bsa Free, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/scp3/sycp3 antibody - bsa free/product/Bio-Techne corporation
Average 93 stars, based on 1 article reviews
scp3/sycp3 antibody - bsa free - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier

Image Search Results


Fig. 2. TAOK2β associates with the polyribosome complex, and its deficiency enhances general translation and protein synthesis. (A) Immunoblots of polysome fractions showing the presence or absence of TAOK2β across all polysome fractions isolated from Taok2+/+ (top) or Taok2−/− (bottom) mouse cortex. PABP1, RPL7a, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were used as positive and negative controls, respectively, to prove polysome preparation efficiency. (B) Polysome profiles from Taok2+/+ and Taok2−/− mouse cortices. (C) Quantification of polysome profiles in Taok2−/−, Taok2+/−, and Taok2+/+ mice cortices. Taok2+/+, n = 13; Taok2+/−, n = 8; Taok2−/−, n = 12; scanning electron microscopy (SEM) error bars, ordinary one-way analysis of variance (ANOVA) (P = 0.0251) followed by Dunnett’s multiple com- parisons test (*P < 0.05). (D) Immunostainings of acute cortical slices from the prefrontal cortex of Taok2+/+ and Taok2−/− mice analyzed by SUnSET assay. WT slices pre- treated with the translation inhibitor cycloheximide (WT + CHX) verifies the specificity of the puromycin antibody signal. (E) Immunoblot from acute cortical slices analyzed by SUnSET assay. (F) Quantification from (E). Taok2+/+, n = 5; Taok2+/−, n = 6; Taok2−/−, n = 5; SEM error bars, ordinary one-way ANOVA (P = 0.0167) followed by Dunnett’s multiple comparisons test (*P < 0.05). (G) Immunostainings of primary neurons (7DIV) analyzed by SUnSET assay. Cultured WT neurons were pretreated with CHX (WT + CHX). (H and I) Quantifications of immunofluorescent SUnSET assay of 7DIV neurons or 21DIV. 7DIV cells: Taok2+/+, n = 236 (five embryos); Taok2+/−, n = 113 (four embryos); Taok2−/−, n = 166 (four embryos). 21DIV cells: Taok2+/+ (three embryos), n = 81; Taok2+/−, n = 73 (two embryos); Taok2−/−, n = 79 (three embryos). SEM error bars, ordinary one-way ANOVA (P < 0.0001) followed by Dunnett’s multiple comparisons test (****P < 0.0001). Scale bars, 10 μm [(D) and (G)].

Journal: Science advances

Article Title: The autism susceptibility kinase, TAOK2, phosphorylates eEF2 and modulates translation.

doi: 10.1126/sciadv.adf7001

Figure Lengend Snippet: Fig. 2. TAOK2β associates with the polyribosome complex, and its deficiency enhances general translation and protein synthesis. (A) Immunoblots of polysome fractions showing the presence or absence of TAOK2β across all polysome fractions isolated from Taok2+/+ (top) or Taok2−/− (bottom) mouse cortex. PABP1, RPL7a, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were used as positive and negative controls, respectively, to prove polysome preparation efficiency. (B) Polysome profiles from Taok2+/+ and Taok2−/− mouse cortices. (C) Quantification of polysome profiles in Taok2−/−, Taok2+/−, and Taok2+/+ mice cortices. Taok2+/+, n = 13; Taok2+/−, n = 8; Taok2−/−, n = 12; scanning electron microscopy (SEM) error bars, ordinary one-way analysis of variance (ANOVA) (P = 0.0251) followed by Dunnett’s multiple com- parisons test (*P < 0.05). (D) Immunostainings of acute cortical slices from the prefrontal cortex of Taok2+/+ and Taok2−/− mice analyzed by SUnSET assay. WT slices pre- treated with the translation inhibitor cycloheximide (WT + CHX) verifies the specificity of the puromycin antibody signal. (E) Immunoblot from acute cortical slices analyzed by SUnSET assay. (F) Quantification from (E). Taok2+/+, n = 5; Taok2+/−, n = 6; Taok2−/−, n = 5; SEM error bars, ordinary one-way ANOVA (P = 0.0167) followed by Dunnett’s multiple comparisons test (*P < 0.05). (G) Immunostainings of primary neurons (7DIV) analyzed by SUnSET assay. Cultured WT neurons were pretreated with CHX (WT + CHX). (H and I) Quantifications of immunofluorescent SUnSET assay of 7DIV neurons or 21DIV. 7DIV cells: Taok2+/+, n = 236 (five embryos); Taok2+/−, n = 113 (four embryos); Taok2−/−, n = 166 (four embryos). 21DIV cells: Taok2+/+ (three embryos), n = 81; Taok2+/−, n = 73 (two embryos); Taok2−/−, n = 79 (three embryos). SEM error bars, ordinary one-way ANOVA (P < 0.0001) followed by Dunnett’s multiple comparisons test (****P < 0.0001). Scale bars, 10 μm [(D) and (G)].

Article Snippet: The following antibodies were used in these studies for immunocytochemical or WB analysis: rabbit anti- Taok2β (Synaptic Systems, 395003; WB, 1:1000; Immunocytochemistry (ICC), 1:250; IP, 1:100); mouse anti- puromycin, clone 12D10 [Millipore, MABE343; WB, 1:10,000; immunohistochemistry (IHC); ICC, 1:5000]; chicken anti- MAP2 (Synaptic Systems, 188006; IHC, 1:500); guinea pig anti–β3- tubulin (Tuj1) (Synaptic Systems, 302304; ICC, 1:500); mouse anti–β- actin (Sigma- Aldrich, A5316; WB, 1:2000); rabbit anti–β- actin (13E5) (Cell Signaling Technology, 4970; WB, 1:5000); rabbit anti–Myc- tag (71D10) (Cell Signaling Technology, 2278; WB, 1:1000; ICC, 1:300); rabbit anti–immunoglobulin G (IgG) (Millipore, 03- 241; IP, 1:200); rabbit anti–phospho- eEF2 (Thr56) (Cell Signaling Technology, 2331; WB, 1:1000); rabbit anti–GSTtag (Cell Signaling Technology, 2625; WB, 1:1000); mouse anti– His- tag (27E8) (Cell Signaling Technology, 2366; WB, 1:1000); rabbit anti–His- tag (Cell Signaling Technology, 2365; WB, 1:1000); mouse anti- eEF2, clone 4B3- G7- H5 (Abcam, ab131202; WB, 1:2000); rabbit anti–phospho- eEF2K (Ser366) (Cell Signaling Technology, 3691; WB, 1:1000); rabbit anti- RPL7a (E109) (Cell Signaling Technology, 2415; WB, 1:1000); rabbit anti- PABP1 (Cell Signaling Technology, 4992; WB, 1:1,000); and mouse anti- eEF2K antibody (C- 12) (sc- 390710; WB, 1:200).

Techniques: Western Blot, Isolation, Electron Microscopy, Cell Culture

Pre‐rRNPs colocalize with TopBP1 at DSBs. A) Pre‐rRNA colocalizes with TopBP1 at DSBs. Following 10 Gy of IR treatment on HCT116 cells, RNA FISH with pre‐rRNA probes and IF with anti‐TopBP1 antibody were performed. B–D) Pre‐rRNA‐associated proteins colocalize with TopBP1 at DSBs. Following 10 Gy of IR, IF was performed with anti‐TopBP1 and anti‐RPL7A (B), or anti‐RPS3 (C), or anti‐FBL antibodies (D). E) Pre‐rRNPs localize at I‐SceI‐induced DSB. I‐SceI‐mediated DSB was induced by Dox and TA treatment in the HCT116 cells harboring a solo I‐SceI site. Then, ChIP assay with the indicated antibodies was performed. Data are represented as mean ± SD as indicated from three independent experiments. F) TopBP1 localizes at the unsynapsed region of the XY body. Meiotic spread was examined with anti‐TopBP1 antibody. Sycp3 was a surrogate marker of the unsynapsed axis of the XY body in pachytene cells. G) Pre‐rRNA localizes at the unsynapsed region. Pre‐rRNA was examined by RNA FISH with pre‐rRNA probes. H–J) Pre‐rRNA‐associated proteins localize at the unsynapsed region. Meiotic spreads were stained with anti‐Sycp3 and anti‐RPL7A (H), anti‐RPS3 (I), or anti‐FBL antibodies (J). The relative intensity of fluorescence signals is analyzed. Two‐tailed Student's t ‐test is used to determine statistical significance. *** p < 0.001, versus control groups. Scale bars, 10 µm.

Journal: Advanced Science

Article Title: Pre‐rRNA Facilitates TopBP1‐Mediated DNA Double‐Strand Break Response

doi: 10.1002/advs.202206931

Figure Lengend Snippet: Pre‐rRNPs colocalize with TopBP1 at DSBs. A) Pre‐rRNA colocalizes with TopBP1 at DSBs. Following 10 Gy of IR treatment on HCT116 cells, RNA FISH with pre‐rRNA probes and IF with anti‐TopBP1 antibody were performed. B–D) Pre‐rRNA‐associated proteins colocalize with TopBP1 at DSBs. Following 10 Gy of IR, IF was performed with anti‐TopBP1 and anti‐RPL7A (B), or anti‐RPS3 (C), or anti‐FBL antibodies (D). E) Pre‐rRNPs localize at I‐SceI‐induced DSB. I‐SceI‐mediated DSB was induced by Dox and TA treatment in the HCT116 cells harboring a solo I‐SceI site. Then, ChIP assay with the indicated antibodies was performed. Data are represented as mean ± SD as indicated from three independent experiments. F) TopBP1 localizes at the unsynapsed region of the XY body. Meiotic spread was examined with anti‐TopBP1 antibody. Sycp3 was a surrogate marker of the unsynapsed axis of the XY body in pachytene cells. G) Pre‐rRNA localizes at the unsynapsed region. Pre‐rRNA was examined by RNA FISH with pre‐rRNA probes. H–J) Pre‐rRNA‐associated proteins localize at the unsynapsed region. Meiotic spreads were stained with anti‐Sycp3 and anti‐RPL7A (H), anti‐RPS3 (I), or anti‐FBL antibodies (J). The relative intensity of fluorescence signals is analyzed. Two‐tailed Student's t ‐test is used to determine statistical significance. *** p < 0.001, versus control groups. Scale bars, 10 µm.

Article Snippet: Detailed information of antibodies used in this study: anti‐Flag (Sigma, Rabbit, F7425), anti‐TopBP1 (Santa Cruz, Mouse, sc‐271043; BETHYL, Rabbit, A300‐111A), anti‐γH2AX (Novus, Rabbit, NB100‐384; CST, Rabbit, 9718), anti‐SCP3 (Santa Cruz, Mouse, sc‐74569; Novus, Rabbit, NB300‐232), anti‐rRNA (Novus, Mouse, NB100‐662), anti‐RPL7A (ABclonal, Rabbit, A13713), anti‐RPS3 (ABclonal, Rabbit, A2533), anti‐FBL (CST, Rabbit, 2639S; Santa cruz, Mouse, sc‐166001), IgG (Rabbit, CST, 2729), IgG (Mouse, CST, 3420), anti‐ATR (BETHYL, Rabbit, A300‐137A), anti‐Chk1 (Novus, Rabbit, NB100‐464), anti‐pATR (T1989) (GeneTex, Rabbit, GTX128145), anti‐pChk1 (Ser345) (CST, Rabbit, 2348), anti‐GAPDH (CST, Mouse, 97 166), anti‐pH3 (Ser10) (CST, Rabbit, 9701), anti‐Puromycin (Millipore, Mouse, MABE343), anti‐GAPDH (HUABIO, Rabbit, ET1601‐4), anti‐Histone H3 (CST, Rabbit, 4499S), anti‐ATM (GeneTex, Mouse, GTX70107), anti‐pATM (S1981) (ROCKLAND, Mouse, 200‐301‐400), anti‐pRPA (Novus, Rabbit, NBP1‐23017), anti‐Rad9 (Santa Cruz, Mouse, sc‐74464), anti‐RAD51 (abcam, Rabbit, ab63801).

Techniques: Marker, Staining, Fluorescence, Two Tailed Test